APPENDIX 375 



SOLUTIONS FOR USE IN STAINING 



Aceton-alcohol for decolorizing in Gram's method of 

 staining: 



Aceton 10 c.c. 



Absolute alcohol 100 c.c. 



Acetic acid-alcohol for clearing in making impression 

 preparations (also used for decolorizing in ordinary method 

 of spore-staining) : 



Alcohol, 90% 2 parts 



Acetic acid, 1% 1 part. 



Mordant for staining flagella: 



Tannin, 20% 10 c.c. 



Ferrous sulphate, cold saturated solution . . 8 c.c. 

 Fuchsin, cold saturated solution in absolute 



alcohol 1 c.c. 



Lugol's iodin solution, for use in Gram's staining method: 



lodin 1 gm. 



Potassium iodid 3 gms. 



Distilled water . . 300 c.c. 



