50 PHYSIOLOGICAL AND PATHOLOGICAL CHEMISTRY. 



IV. Solution of the Blood-corpuscles. 



Treat a small portion of the blood in a test-tube with some 

 ether and some water, and shake thoroughly: the blood-cor- 

 puscles dissolve, the blood becomes transparent (laky blood). 

 A solution of the salts of the bile-acids acts in the same way 

 on the blood. 



V. Preparation of Crystallized Oxyhaemoglobin. 



The preparation of crystallized oxyhsemoglobin is readily 

 accomplished only with certain kinds of blood (dog, horse, 

 guinea-pig, rat), but not with the blood of man, ox, pig, or 

 rabbit. 



One hundred cubic centimeters of dog's blood are shaken 

 vigorously in a flask with air, cooled to 0, shaken with 10 cc. 

 of ether and 10 cc. of water, so that the blood-corpuscles dis- 



FIG. 3. Crystals of Oxyhsemoglobin from Dog's Blood. 



solve and the blood becomes laky (the complete solution of 

 the blood-corpuscles is to be determined by means of a micro- 

 scopical examination), and the mixture allowed to stand 

 at 0. The separation of the oxyhsemoglobin crystals takes 

 place at once or after a few hours when dog's blood is used. 



