CHAPTER V. 

 PATHOLOGICAL TRANSUDATES, CYSTIC FLUIDS. 



I. Examination for Coagulable Albumins. 



II. Examination for Proteids Precipitated by Acetic Acid and In- 

 soluble in Excess of the Reagent. 



III. Examination for Albumin and Globulin. 



IV. Examination for Urea. 

 V. Examination for Sugar. 



VI. Examination for Paralbumin. 



I. Examination for Coagulable Albumins. 



See in this connection the chapter on Blood, page 48. It 

 is to be noted that in case of strongly alkaline transudates 

 containing but little albumin no coagulation may result on 

 heating. In such cases the addition of the acetic acid after 

 the heating must be done exceedingly carefully, and in case 

 no precipitate appears it is advisable to add 1 to 2 cc. of con- 

 centrated salt solution. 



II. Proteids Precipitated by Acetic Acid. 1 



To about 100 cc. of the clear fluid add acetic acid until the 

 reaction is distinctly acid ; if a precipitate insoluble in excess 

 of the reagent is formed, the fluid contains mucin or nucleo- 

 albumin. Filter off the precipitate, wash, then grind the 



1 In case pathological fluids are not available, prepare an extract from 

 the thymus gland of the calf (1 part of the finely divided gland treated 

 with ten parts of cold water for 24 hours and shaken from time to time 

 and then filtered), and mix this with an equal volume of blood-serum or 

 ascitic (dropsical) fluid. 



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