CHAPTER VII. 

 EXAMINATION OF THE PANCREAS. 



I. Tryptic Digestion. 

 II. Diastatic Action. 



III. Cleavage of Fats. 



IV. The Nucleoproteid of the Pancreas. 



I. TRYPTIC DIGESTION. 



Digest 250 g. of fibrin with 1 liter of alkaline chloroform-water and 2 

 to 2.5 g. of pancreas-powder for 48 hours, add acetic acid, boil, and filter. 



| 



Residue: coagulated albumin. Filtrate (A) (reaction with bro- 



mine), evaporate, and let stand. 



r ~i 



Crystals (B) : tyrosine. Filtrate (C) evaporate further: 



leucine, peptone 



The best material is fresh fibrin. If only .the coagulate " material 

 preserved in chloroform-water is available, it is advisable ,o heat it 

 with acidified water (3 cc. of hydrochloric acid, specific gravity 1.19, 

 to 1 liter of water), and then wash it thoroughly on a muslin filter. 

 Sometimes this treatment is omitted. The digestion takes place with 

 the unswollen fibrin also, but not so well. The chloroform-water is 

 prepared by shaking 1 liter of water with 5 cc. of chloroform. The 

 chloroform is used to prevent putrefaction: thymol may also be used 

 for this purpose. If antiseptic material is not used, very marked pu- 

 trefaction is certain to result. The pancreas-powder is prepared 

 according to the method of Kuhne. Pancreas 1 which has lain for 

 twenty-four hours is carefully freed from all visible fat, ground with 

 absolute alcohol, filtered after standing a short time and pressed, 



1 From the ox or dog. 



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