CHAPTER VIII. 

 EXAMINATION OF BILE. 



I. Detection of the Constituents of Bile. 

 II. Detection of Mucin in Bile. 

 III. Preparation of Taurine. 



I. DETECTION OF THE CONSTITUENTS OF BILE. 



Mix 200 cc. of ox-bile with bone-black, evaporate to dryness, heat with 

 absolute alcohol, and filter. 



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Residue : mucin, salts, and Alcoholic solution add ether, 



bone-black. 



Precipitate : salts of the bile-acids. Solution contains cholesterin. 



Two hundred cubic centimeters of ox-bile are evaporated 

 as nearly to dryness as possible on the water-bath with some 

 good bone-black (one-fourth the volume), the residue removed 

 from the dish after cooling, put into a flask, and extracted on 

 the water-bath with alcohol. After cooling, filter, evaporate 

 the extract to dryness on the water-bath, dissolve the dry 

 mass in absolute alcohol, and filter into a dry flask. Add to 

 the nitrate anhydrous ether until the cloudiness becomes 

 permanent . After long standing, sometimes even on the next 

 day, a mixture of the sodium salts of glycocholic acid, 

 C 26 H 43 N0 6 , and taurocholic acid, C 2fl H 45 NS0 7 , crystallizes 

 (Plattner's crystallized bile). 



The bile-acids are distinguished by a reaction which is 



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