EXAMINATION OF ADIPOSE TISSUE. 149 



2. Try the acrolein test with a few drops of the glycerin 

 '(see page 142). 



3. Dilute the rest of the glycerin with water (clear solu- 

 tion), add a little sodium hydroxide and then a few drops of 

 copper sulphate. The cupric hydroxide, which first sepa 

 rates, dissolves, forming a deep-blue fluid. This solution does 

 not give any cuprous oxide on heating, but remains unchanged 

 (distinction from many sugars, especially glucose). 



a small Bunsen flame for a few minutes, add a little alcohol, and ignite. 

 The alcohol burns with a yellow flame (due to the sodium in the borax) 

 in which the green color (due to the boric acid glycerin ester) may b<3 

 plainly seen. The object of heating the glycerin and borax previous to 

 the addition of the alcohol is for the purpose of forming the glycerir. 

 .borate. O. 



