156 PHYSIOLOGICAL AND PATHOLOGICAL CHEMISTRY. 



are poured back on the filter till they run through clear. Test 

 the reaction and repeat the experiments described under 

 blood-serum (see chapter on Blood, page 60). The reac- 

 tions of egg-albumin are very similar to those of the dilute 

 serum; they differ from them, however, in some particulars. 



1. On heating to boiling, the solution becomes milky and 

 the turbidity is more pronounced than in the case of serum 

 solution; a separation of coagulated albumin, however, does 

 not take place. The cautious addition of acetic acid brings 

 about the coagulation. The precipitate is not so flocculent 

 as in the case of serum albumin, but appears somewhat 

 swollen. A further addition of acetic acid dissolves the pre- 

 cipitate, but not so readily as in the case of serum albumin. 1 

 Repeat experiments 2, 3, and 4, under Blood-serum, with the 

 egg-albumin solution. 



5. Heat a portion of the solution with half its volume of 

 sodium hydroxide solution: alkali albuminate is formed. 

 Neutralize the cooled solution with dilute sulphuric or acetic 

 acid: the albuminate precipitates. In excess of the acid 

 this dissolves far more difficultly than in the case of serum 

 albumin. 



6 and 7 as with the serum. 



8. If a portion of the solution is treated with nitric acid 

 till a permanent precipitate is formed and then absolute 

 alcohol is added till the volume has been doubled, the pre- 

 cipitated albumin does not dissolve, or dissolves only very 

 slightly (distinction from serum albumin). 



9. If strong nitric acid of the specific gravity 1.48 be 

 added to a little of the solution, a precipitate is formed which 

 does not dissolve when the quantity of the nitric acid added 

 amounts to about half of the volume of the albumin solution 



1 The expression serum albumin is here used only for the sake of brev- 

 ity in place of "proteids of the blood-serum" 



