PUTREFACTION PRODUCTS OF PROTEIDS. 161 



mixture of phenol and cresol, principally paracresol. In 

 order to show the presence of these substances, heat the oil 

 in a flask with some water and let cool. 



1. Add some ferric chloride to a portion of the solution, 

 dirty bluish-gray color. 



2. Warm a second portion of the solution with some 

 Millon's reagent : red color. 



3. Add to a third portion some bromine- wa ter ; precipitate 

 of tribromphenol and tribromcresol (or other bromine com- 

 pounds) . 



For more detailed information concerning phenol and 

 cresol see pages 106 and 171. 



The aqueous fluid (F) is again placed in the separating- 

 funnel, strongly acidified with hydrochloric acid, and extracted 

 with a small quantity of ether (caution on account of the car- 

 bon dioxide set free). The ethereal solution when drawn off 

 and evaporated leaves the volatile fatty acids r mixed with a 

 small quantity of the homologues of benzoic acid. 



(6) Treatment of the Distillation Residue. 



The distillation residue is evaporated, precipitated with alcohol, and filtered. 



Filtrate (A) evaporated, treated Residue (B) : undissolved albumin, 



with sulphuric acid and ether. bacteria, albumoses, peptone r 



| and salts. 



Ether extract (C) distilled and Aqueous solution (D) contains al- 



treated with sodium hydroxide bumoses and peptone, 

 and barium chloride solutions. 



r ~i 



Precipitate (E) : barium soaps. Filtrate (F) evaporated and treated 



with HC1 and ether. 



Ether solution (G) evaporated, the resi- Aqueous solution (H) contins sodi- 

 due boiled with water, and filtered. um chloride and other chlorides. 



Solution (I) contains skatol-car- Insoluble oil (K) , ground with zinc 

 bonic acid, aromatic oxyacids, oxide, then boiled and filtered: 



and succiriic acid. zinc salts of phenyl-propionic and 



phenyl-acetie acids crystallize. 



