THE URINE. 195 



\CaO), and weigh. This weight multiplied by 1.61 gives the 

 quantity of the oxalic acid (C 2 H 2 4 ). After the weighing, 

 dissolve the lime in a little dilute nitric acid : there should be 

 no evolution of carbon dioxide. The solution obtained is 

 tested with ammonium molybdate for phosphoric acid. 



VHI. DETERMINATION OF PHENOL OR CRESOL. 



Proceed as directed in the qualitative detection of these 

 substances, page 108. Add bromine-water to the distillate 

 until a permanent yellow color results, let stand a few days, 

 filter through a weighed filter which has been dried in a desic- 

 cator over sulphuric acid, dry in the dark in the desiccator 

 over sulphuric acid until the weight is approximately con- 

 stant, and weigh. 331 parts of the precipitate correspond 

 to 94 parts of phenol or 108 of cresol. 



The object of letting the precipitate stand is for the pur- 

 pose of converting the tetrabromcresol, C 6 H(CH 3 )Br 3 OBr, 

 which first forms into tribromphenol. 1 



IX. DETERMINATION OF ALBUMIN. 



One hundred cubic centimeters or, in case of urines 

 containing a large amount of albumin, only 50 cc. of the 

 previously filtered and perfectly clear urine is placed in a 

 beaker, which should be only half-filled by the liquid, a 

 drop of acetic acid is added in case the reaction is not dis- 

 tinctly acid, and the mixture is heated for half an hour in the 

 water-bath, so that the beaker is surrounded by the water, 

 until the coagulum becomes coarsely flocculent. The water- 

 bath should not be too hot in the beginning. If the albumin 

 does not become coarsely flocculent, add a few more drops 

 of acetic acid. Filter through a filter dried at 110-115 



1 For the titration method of Kossler and Penny for determining 

 phenol see C. Neuberg, Zeitschr. f. physiol. Chemie, 28, 123. 



