24 LABORATORY METHODS OF UNITED STATES ARMY 

 OXALATE SOLUTION FOR BLOOD CULTURE. 



Ammonium oxalate 2.0 grams 



Sodium chloride ...... 6.0 grams 



In distilled water 1000.0 c.c. 



ZENKER'S FLUID. 

 Potassium dichromate .... 2.5 grams 



Mercury bichloride 5.0 grams 



Water 100.0 c.c. 



Glacial acetic acid 5 per cent, is added to this stock solu- 

 tion just before use. 



COPPER SULPHATE SOLUTION FOR CAPSULE STAIN. 



20 grams of copper sulphate crystals are dissolved in 100 c.c. 

 of water. 



STOCK STAINING SOLUTIONS. 



It is convenient in stationary laboratories to keep stock 

 stains in the form of saturated solutions. The strengths 

 of various saturated solutions are as follows: 



Saturation strengths. 



Fuchsin in alcohol 3.0 per cent. 



Gentian violet in water . . . . 1.5 " 

 Gentian violet in alcohol . . . 4.8 

 Methylene blue in water . . .6.7 " 

 Methylene blue in alcohol . . . 7.0 " 



Safranin . .4.0 



Saturated alcoholic solutions can be kept and aqueous 



staining solutions can best be made by adding 5 per cent, of 



the filtered alcoholic solutions to water. 



LOEFFLER'S ALKALINE METHYLENE BLUE. 



Saturated alcoholic solution of methyl- 



ene blue 30.0 c.c. 



i to 10,000 solution potassium hydrox- 

 ide in water i oo. o c.c. 



