SOLUTIONS AND STAINS 25 



ZIEHL CARBOL-FUCHSIN SOLUTION. 



Fuchsin (basic) i.ogram 



Alcohol (absolute) 10 . o c.c. 



5 per cent, phenol loo.oc.c. 



To make up this staining solution by another method, 

 90 c.c. of a 5 per cent, aqueous solution of phenol is mixed 

 with 10 c.c. of saturated alcoholic basic fuchsin. 



CAPSULE STAINS. Hiss's Copper Sulphate Method. Cover- 

 slip preparations are made by smearing the organisms in a 

 drop of animal serum, preferably beef-blood serum. 



Dry in air and fix by heat. 



Stain for a few seconds with 



Saturated alcoholic solution of fuchsin or gentian violet, 

 5 c.c., in distilled water, 95 c.c. This combination is often 

 too weak for good results. A gentian-violet or fuchsin 

 solution twice as strong is advantagenous. Gram's gentian 

 violet or carbol-fuchsin can be used. 



The cover-slip is flooded with the dye and the preparation 

 held for a second over a free flame until it steams. 



Wash off dye with 20 per cent, aqueous copper sulphate 

 solution. 



Blot (do not wash). 



Dry and mount. 



NEISSER STAIN FOR POLAR BODIES. 



Methylene blue i . o gram 



Absolute alcohol 200.0 c.c. 



Glacial acetic acid . . . ' . . 50 . o c.c. 



Distilled water looo.oc.c. 



Preparations fixed by heat are immersed in this stain for 

 five seconds and then washed in water and counterstained 

 with Bismarck brown or, better, safranin. 



