30 LABORATORY METHODS OF UNITED STATES ARMY 



by Romanowsky, and use only distilled water or rain water, 

 in all Romanowsky methods. Old distilled water should 

 be boiled to drive off CO 2 , especially for Giemsa stain. 



FONTANA METHOD FOR TREPONEMATA. 

 The following three solutions are used: 



I 



Acetic acid i . o 



Formalin 20.0 



Distilled water 100.0 



II. 



Phenol i.o 



Acid tannic 5.0 



III. 



Twenty-five per cent, solution of silver 



nitrate 5.0 c.c. 



Ammonia water i.o drop 



Dry slide in air. 



Wash in I for one minute. 



Wash in water. 



Pour on II and steam one-half minute. 



Wash in water. 



Pour on III, steam for one-half minute. 



Wash in water. 



Mount in balsam. 



Immersion oil takes out the color. 



CLEANSING SOLUTION FOR GLASSWARE. 



Potassium bichromate (powdered) . 200 grams 

 Water distilled, up to .... 1500 c.c. 

 Sulphuric acid cone 500 c.c. 



This solution may be used for cleaning test-tubes and other 

 glassware used in the preparation of the Colloidal Gold 



