SOLUTIONS AND STAINS 31 



Reagent and the Lange test. Microscopic slides cleaned with 

 this solution may be used repeatedly. 



Method for preparation of chemically clean glassware: 



1. Boil for thirty minutes in hot Ivory soap solution. 



2. Brush thoroughly under hot tap water. 



3. Rinse in running water for ten minutes. 



4. Immerse in hot bichromate solution for thirty minutes. 



5. Rinse in running water for ten minutes. 



6. Rinse in ordinary distilled water. 



7. Rinse with triply distilled water. 



KLOTZ'S FLUID. 



The chief advantage of Klotz's method of preserving 

 pathological specimens is that the specimens may be preserved 

 and shipped in Fluid No. i. 



A practical method for a Base Hospital with a large patho- 

 logical service is to make up a liberal supply of Carlsbad 

 salts with which the fluid is readily prepared. 

 Modified Carlsbad salts: 



Potassium sulphate 40 grams 



Sodium or potassium nitrate . . . 760 grams 



Sodium chloride 360 grams 



Sodium bicarbonate 400 grams 



Sodium sulphate 440 grams 



Fix tissues three to five days or longer in Fluid No. i: 



Carlsbad salts 375 grams 



Chloral hydrate . . . - . . . 375 grams 



Formalin 375 c.c. 



Water 15 liters 



Wash three to five hours in running water. Preserve in 

 Fluid No. 2 : 



