32 LABORATORY METHODS OF UNITED STATES ARMY 



Carlsbad salts 375 grams 



Chloral hydrate 150 grams 



Formalin 75 c.c. 



Water 15 liters 



KAISERLING'S FLUID. 



For preservation of colors of gross pathological specimens 

 the following method gives satisfactory results: 



1. Fix the tissue in following solution for one to five days 

 in Kaiserling's Fluid No. i : 



Formaldehyde 200 c.c. 



Water 1000 c.c. 



Potassium nitrate 15 grams 



Potassium acetate 30 grams 



2. Drain and place in 80 per cent, alcohol for one to six 

 hours. 



3. Ninety- five per cent, alcohol for one to two hours. 



4. Preserve specimen in Kaiserling's Fluid No. 3: 



Potassium acetate . . . . . 200 grams 



Glycerine 400 c.c. 



Water 2000 c.c. 



NEUTRAL SODIUM HYPOCHLORITE SOLUTION 

 ("DAKIN'S SOLUTION"). 



PREPARATION FROM BLEACHING POWDER. Dakin's Origi- 

 nal Method. A strong solution of hypochlorite is prepared 

 by decomposing 150 grams bleaching powder (about 25 to 

 35 per cent, available chlorine) with 105 grams dry sodium 

 carbonate (122 grams monohydrate (Na 2 CO3.H 2 0) or 284 

 grams washing soda (Na 2 CO3.ioH 2 0) ). The mixture is very 

 thoroughly shaken, both to make good contact and to render 



