SOLUTIONS AND STAINS. 



PHYSIOLOGICAL SALT SOLUTION. 



FOR bacteriological work, physiological solution is usually 

 made up by adding 8.5 grams of sodium chloride to a liter 

 of distilled water. When for reasons of speed and convenience 

 tap water is used, one should have some idea of the salt 

 contents of the water used before relying upon it. 



SODIUM CITRATE SOLUTION. 



For bacteriological purposes this contains i per cent, of 

 sodium citrate and 0.85 per cent, of sodium chloride. 



If sodium citrate is to be used for prevention of coagulation 

 of blood without considerably changing the volume of the 

 blood the solution is made up to contain 10 per cent, of 

 sodium citrate and 0.85 sodium chloride. 



FIVE PER CENT. SULPHURIC ACID FOR DECOLORIZING 

 (AS USED IN TUBERCLE BACILLUS STAINING). 



Slowly allow 2.7 c.c. of c. p. sulphuric acid of a specific 

 gravity of 1.84 to flow into 80 c.c. of distilled water. After 

 cooling, bring volume up to 100 c.c. (St. Luke's Manual.) 



ACID ALCOHOL (ORTH). 



HC1 I.OC.C. 



70 per cent, alcohol 99.0 c.c. 



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