20 LABORATORY METHODS OF UNITED STATES ARMY 



tive complement-fixation test may be obtained from the 

 seventh to the tenth day and usually persists during the 

 course of the disease. The agglutination test may be positive 

 in four to seven days, the content in agglutinins increasing 

 early in the disease, but decreasing if the disease becomes 

 chronic. All blood-serum tests are influenced by the injec- 

 tion of mallein or vaccine, and blood should be taken before 

 their administration. 



|t Blood for these tests is collected from man as per instruc- 

 tions under Wassermann, page 16; from horse as follows: 



(a) Sterilize a large-sized hypodermic needle by boiling. 

 Do not use phenol or other antiseptics for this purpose. 



(b) Shave and sterilize with tincture of iodin the skin over 

 the jugular vein. 



(c) Make the vein prominent by pressing with the thumb 

 below the area selected for puncture, thrust needle into vein 

 and permit blood to flow into sterile bottle furnished for this 

 purpose. Tightly cork after collection. 



Stand toward the side of the horse, while drawing blood, 

 to avoid danger from sudden rearing. 



Label specimen plainly with name (human case) or name 

 or identification mark of animal, the nature of the specimen, 

 the test desired and the name, rank and station of the surgeon 

 or veterinarian to whom report is to be returned. Pack the 

 bottle securely in cotton to avoid breakage. Requests in 

 duplicate, on Form 5$u, M. D., must accompany each speci- 

 men. 



WATER ANALYSIS, BACTERIOLOGICAL. DIRECTIONS FOR 

 COLLECTING AND SHIPPING. 



These directions are a transcription of the instructions in 

 the Manual of the Medical Department, 1916: 



356. At the time of forwarding the water the officer to 

 whom it is sent should be advised of the following particulars: 

 (i) The date, place and mode of shipment; (2) the date 



