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LABORATORY MANUAL 



Apparatus 



Wide mouth bottles 

 Rubber stoppers 

 Blotting paper 

 Thermometer 



Soaked seeds, peas, corn, etc. 



Refrigerator 



Oven 



Directions: A. Relation of temperature to germi- 

 nation. 



In the bottom of each of the four wide mouth bot- 

 tles, place several layers of thoroughly moistened 

 blotting paper. In each bottle put twelve soaked pea 

 seeds. With the conditions of moisture, light, and air- 

 supply the same for all bottles, place them in different 

 but fairly constant temperatures. Place : 



1. the first bottle on the ice in the refrigerator ; 



2. the second bottle on the shelf in the refrigerator ; 



3. the third bottle in the room ; 



4. the fourth in an oven at a temperature of 100 F. 

 With a thermometer obtain and record the exact 



temperature in each instance. Make daily observa- 

 tions for a week, tabulating your results as follows : 



What do you conclude from the above experiment 

 is the best temperature for the germination of peas? 

 B. Relation of moisture to germination. 

 Place seeds in four wide mouth bottles as follows: 



