TABLE OF CONTENTS 



PAGE 



CHAPTER I. Laboratory Rules . . . . . . i 



CHAPTER II. Cleaning, Preparing, and Sterilizing Glassware 8 



CHAPTER III. Preparation of Culture Media . . .11 



Exercise i. Preparation of nutrient agar-agar . .11 



Exercise 2. Preparation of glucose-agar . . -19 



Exercise 3. Preparation of peptone-gelatin . -23 



Exercise 4. Preparation of peptone-broth . -25 



Exercise 5. Preparation of potato . . . .27 



Exercise 6. Preparation of litmus milk . . .28 



CHAPTER IV. Preparation of Staining Solutions . . 30 



CHAPTER V. The Microscope . . . . -31 



CHAPTER VI. Collecting Bacteria from the Air . . -37 



CHAPTER VII. Exercises on Infection and Sterilization . 45 



Exercise i. Phenomena of infection . . -45 



Exercise 2. Phenomena of sterilization . . -45 



Exercise 3. Phenomena of sterilization (continued) . 45 



CHAPTER VIII. Study of Yeasts, Molds, and Torulae . 49 



Exercise i. Cultural studies . . . . -49 



Exercise 2. Study of the germination of spores . 51 



CHAPTER IX. Scheme for Routine Study of the Various 



Groups of Organisms . . , . . -53 



CHAPTER X. Method of Describing Cultures . . -57 



CHAPTER XI. Study of Certain Chromogenic Bacteria . 69 



Exercise i. Cultural studies . ' . . . .69 



Exercise 2. Study of pigments . . . . 71 



CHAPTER XII. The Pyogenic Group . . -73 



Exercise i. Subgroup A . . . . -73 

 Exercise 2. Subgroup B . .... 76 



