

CHAPTER I 

 LABORATORY RULES 



1. Carefully familiarize yourself with the laboratory 

 rules. Upon their careful observance depend good work 

 and your own safety. 



2. Food must not be eaten in the laboratory; lead- 

 pencils, labels, or fingers must not be moistened with 

 the tongue. 



3. If any portion of a culture is spilled by accident 

 upon the desk or floor, it should be immediately covered 

 with a germicide (HgCl 2 i : 1000 or 5 per cent, carbolic 

 acid). After this has acted for 10 or 15 minutes, wipe it 

 up and throw the cloth or paper into a waste-jar. 



4. In case the hands should by accident come in 

 contact with infectious material, they should be washed 

 with one of the above-mentioned germicides, and then 

 thoroughly scrubbed with soap and water. 



5. The platinum needles used in making cultures 

 should be sterilized in a flame shortly before and imme- 

 diately after use, and before they are laid down. When 

 the needles are covered with moist infectious material, 

 they should be held at the side of the flame until dry 

 before being sterilized; this will avoid the danger of 

 scattering this material about the desk. 



6. All possible care should be observed in the care 

 of the apparatus, desk, etc. Solid materials must not 

 be put into the sinks. Burned matches, paper, cotton, 

 etc., are to be put in the crocks provided for that purpose, 

 and not on the floor. 



