2 LABORATORY GUIDE IN BACTERIOLOGY 



7. Discarded cultures should be killed in the auto- 

 clav (5 minutes at I2o 0j ) before emptying into the crocks. 



8. When using the steam sterilizers and autoclav, 

 see that the pan contains sufficient water before lighting 

 the gas. 



9. In general, see that the air inlets of Bunsen burners 

 are open before lighting, and relight if the flame strikes 

 down. 



10. Always return stock-bottles to the proper places 

 on the shelves. 



11. At the close of the day's work the desks should 

 be washed off with corrosive sublimate, and the hands 

 cleaned by thorough scrubbing with green soap and 

 water. 



12. Before leaving the laboratory, see that the gas 

 is shut off under all apparatus, that water faucets are 

 closed, and that all glassware, etc., is replaced in the 

 lockers. 



GENERAL DIRECTIONS 



1. After obtaining the key to the locker, examine the 

 outfit, check the apparatus (Fig. i) on the furnished list, 

 and return the same signed with your name. This signa- 

 ture is a receipt in full for the material received and in 

 good condition, and the student is personally responsible 

 for the return of the same in good condition. 



2. Matches, peptone, gelatin, and filter-paper are 

 furnished with the locker outfit in sufficient quantities 

 to last through the course. Anything needed in excess 

 must be furnished by the student. 



1 Degrees of temperature throughout this work are indicated 

 according to the Centigrade system. 



