ioo LABORATORY GUIDE IN BACTERIOLOGY 



culture in a "pocket" under the skin. When the animal 

 is dead, perform an autopsy, and observe particularly the 

 hemorrhagic and gelatinous edema under the skin; also 

 the enlarged spleen and the hemorrhagic adrenals. 

 Make a stained preparation from the heart's blood, and 

 observe the lack of spores, and also the presence of 

 capsules and degenerate forms, which do not stain. 

 What prevents the formation of spores ? How would 

 you distinguish B. anthracis from B. subtilis? 



