i 4 LABORATORY GUIDE IN BACTERIOLOGY 



weight of the different ingredients, allowing 1000 g. for 

 all the water used and 30 g. for the weight of the white of 

 each egg and the weight of the pan. If the actual total 

 weight is in excess of the amount calculated, boil again 



a 



FIG. 5 



Apparatus for Filtering 

 Media 



a. Filter 



b. Large funnel 



c. Small funnel 



d. Rubber hose 



e. Pinchcock 

 /. Pipette 



g. Culture-tube 



until the proper weight is reached. If, on the other hand, 

 the total weight is less, make up the deficiency by adding 

 water. 



Example 



TVater 



Agar 



Extract of beef 



Peptone 



Whites of two eggs 



Saucepan for example . . 



Total. 



