62 LABORATORY GUIDE IN BACTERIOLOGY 



2. Size: approximate estimate in microns. 

 Note whether large or small, thick or slender, 

 round or square ends, etc. 



3. Arrangement of bacteria: in groups, chains, 

 bunches, pairs (diplococcus) , sarcina form, 

 threads, branching, etc. Also note different 

 arrangements, if observed, in different media. 



4. Staining powers: It is sufficient to mark + 

 for positive, - - for negative stains. Special 

 stains must be described more fully. 



5. Motility: If absent, mark ; if present, +. 

 In the latter case describe the character of 

 the movement. 



6. Spores: Absence noted by ; presence, by 

 + . This information is to be gathered from 

 the textbook, unless specially instructed. 

 If so mention the method of spore-stain applied. 



7. Note any peculiar appearance in the micro- 

 scopical picture, especially involution forms 

 and the presence or absence of capsules. If 

 capsules are present, note the method of 

 demonstration. 



V. Physiological characters. 



1. Relation to temperature : What is the optimum 

 temperature? (See textbook or references.) 



2. Relation to free oxygen: aerobe, anaerobe, 

 facultative aerobe or anaerobe. 



3. Relation to disinfectants, light, desiccation, 

 heat (thermal death-point). (Refer to text- 

 book or references.) 



4. Pigment-production: If present, +; if absent, 

 . In the former case, note the color, dif- 



