76 LABORATORY GUIDE IN BACTERIOLOGY 



8. Remove the anterior thoracic wall by cutting away 

 the ribs from below upward on each side to the thoracic 

 apex. 



The viscera are now thoroughly exposed. Cultures 

 and smears should be made from different parts, 

 especially from the heart's blood, peritoneal cavity, 

 spleen, liver, and localized foci of suppuration. 



EXERCISE II. THE PYOGENIC GROUP (SUBGROUP B) 



MEMBERS 



Micrococcus lanceolatus. 

 Micrococcus gonorrhoeae. 

 Micrococcus intracellularis meningitidis. 

 Micrococcus zymogenes. 



Prepared cover-slips of Micr. gonorrhoeae are furnished. 

 Stain these with methylene-blue and Gram's method, 

 study, and describe the microscopical appearance. This 

 organism is extremely difficult to cultivate. It is a strict 

 parasite, and requires special media, and a great amount 

 of time and care, for artificial cultivation. For these 

 reasons, only the morphology, as it appears in gonorrheal 

 pus, and which is very characteristic, is studied. 



Inoculate agar-slants from stock-cultures of Micr. 

 lanceolatus and Micr. zymogenes. 



REFERENCES (Micr. zymogenes) 



MacCallum and Hastings, Journal of Experimental Medi- 

 cine, Vol. IV (1899), p. 521. 



Harris and Longcope, Centralblatt fur Bakteriologie und 

 Parasitenkunde, Part I, Vol. XXX, No. 9 (printed in 

 English). 



1. Routine study. Note particularly the microscopic 

 appearance of both organisms and the action of Micr. 

 zymogenes on milk and gelatin. 



2. Special study A. The staining of capsules from 



