78 LABORATORY GUIDE IN BACTERIOLOGY 



3. Special study B. Inoculation of mouse with Micr. 

 lanceolatus. 



a) Fasten the mouse in the holder (Fig. 32). 



b) Shave a place on the back immediately above the 

 tail. 



c) Wash with a solution of mercuric chlorid (i : 1000), 

 followed by alcohol. 



FIG. 32 

 Mouse-Holder 



d) Inject 0.2 c.c. of a milk-culture of Micr. lanceo- 

 latus. 



e) When dead, perform an autopsy, and study the 

 lesions in the usual manner. 



/) Make cultures in milk and on slant-agar from 

 the heart's blood or the spleen. 



g) Make a capsule stain from the heart's blood, 

 spleen, or other organs. 



