APPENDIX I 

 SPECIAL MEDIA 



STANDARD METHOD OF PREPARING BROTH, NUTRIENT 



GELATIN AND NUTRIENT AGAR 

 (Report of American Public Health Assocciation,Vo\. XXX, 1905.) 



9. Heat over boiling water (or steam) for 30 minutes. 

 10. Restore loss by evaporation. 



u. Titrate after boiling one minute to expel carbonic acid. 

 12. Adjust reaction to + i.o % by adding normal hydrochloric acid or sodium 

 hydrate as required. 



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