SPECIAL MEDIA 131 



Lactose 30 g. 



Litmus-solution 260 c.c. 



Crystal violet (o. \% aqueous solution) 20 c.c. 



Tube and sterilize once in Arnold. 



Uschinsky's non-proteid medium (FraenkePs modifica- 

 tion) . 



Disodium hydrogen phosphate 20 g. 



Ammonium lactate 63 g. 



Asparagin 34 g. 



Sodium chlorid 50 g. 



Water 10,000 c.c. 



Phenol media. i part carbolic acid is added to 1000 

 parts medium. 



Dog-blood serum. Serum of dog's blood is coagulated in 

 slanted tubes in the Koch inspissator for 3 hours at 75. 



Nitrate broth. Add 5 parts of potassium nitrate to each 

 1000 parts of ordinary broth. 



Nitrate solution. 5 c.c. of 2 per cent, aqueous potassium- 

 nitrate solution are added to a solution of i g. of peptone in 

 1000 c.c. of water. 



