APPENDIX II 

 STAINING SOLUTIONS 

 Delafield's hematoxylin 



Hematoxylin crystals 4 g. 



Alcohol .25 c.c. 



Ammonia alum 50 g. 



Water 400 c.c. 



Glycerin 100 c.c. 



Methyl alcohol 100 c.c. 



Alum-hematoxylin 



1 . Hematoxylin 2 g. 



Absolute alcohol 100 c.c. 



2. Ammonia alum 2 g. 



Water 100 c.c. 



Mix i and 2 and add 



Glycerin 850 c.c. 



Glacial acetic acid 100 c.c. 



Allow to stand for one month before using. 

 Bismarck brown 



Bismarck brown o . 5 g. 



Water 100 c.c. 



Sajranin 



Safranin 0.5 g. 



Water 100 c.c. 



Carbolic thionin-blue (Nicolle') 



Thionin-blue i g. 



Carbolic acid 2 . 5 g. 



Water 100 c.c. 



Alum carmin 



Alum 2 . 5 g. 



Carmin i o g. 



Water 100 c.c. 



132 



