FROST'S CULTURE CHART 



4. Pigment production 



5. Growth in carbohydrate media: 

 a. Stab or shake culture 



6. Fermentation-tube: i. Growth in bulb. . . .2. In closed arm. . 



c. Percentage gas produced in: Dextrose. .. .Lactose . . .Saccharose 



After 24 hours. . 



After 48 hours 



TT 



Gas formula T^T~ 



CU 2 



d. Reaction in bulb 2. Closed arm 



6. Acid or alkali production, in litmus milk 



7. Reduction of nitrates; to nitrites to ammonia 



8. Indol production; 24 hours 48 hours 4 days 



fecal odor; 24 hours 48 hours 4 days 



9. Enzym production; coagulative proteolytic diastatic 



10. Characteristic odor 



11. Pathogenesis 



CULTURAL CHARACTERS OF: 



(I) 



Gelatin 

 plate or 

 roll tube 



a) Surface 

 colonies 



b) Deep 

 colonies 



(2) 



Agar plate, 



or roll 



tube 

 a) Surface 



colonies 

 6) Deep 



colonies 



Reaction of 



Medium 

 Incubation 

 Temp. (C) 



Description 



Sketches 



