BACTERIA 95 



sists simply of a swelling of the spore, then the formation of a small rod 

 which issues from the spore and forms a septum for itself (Fig. 73, 15 

 and 1 6). As soon as the spore germinates, the nucleus ceases to exist 

 as a morphologic entity; it is scattered in the cytoplasm in the form of 

 little grains. 



~v -: ':: r. 



Iff I 



10 II IZ 13 14 



FIG. 73. Bacillus butschlii. 1-16, Vegetative cells and their division. 7-9, Begin- 

 ning of sporulation: the cells about to sporulate are partitioned off crosswise; then 

 the septum thus formed is absorbed, at which time sporulation begins. Schaudinn 

 considers this partitioning off followed by fusion of the two daughter cells as a rudi- 

 mentary sexuality. 10-13, Formation of the beginnings of the two spores, at the 

 poles of the cell. 14, Ripe spores. 15-16, Germination of the spore. (After 

 Schaudinn.) 



In another bacillus smaller in size (B. sporonema), Schaudinn has 

 found an analogous structure only at the time of sporulation; he does 

 not prove the formation of an axial filament but only the condensation 

 of a portion of the chromatic grains into a large granule which forms the 

 beginning of the spore (Fig. 74). 



By the fact that in these two bacilli the beginning of the spores 

 appears as a granule equivalent in some respects to a nucleus and 

 resulting from the condensation of a portion of the stainable grains, 

 Schaudinn is led to believe that these grains are composed of chroma tin 

 and represent a kind of diffuse nucleus. 



