PLATE XXVIII. 

 Fio. 1. ANTHRAX BACILLUS. 



Agar culture, incubated at 87 C.; of 24 hours' age : the films treated with 20 per 

 cent, acetic acid before staining with aqueous solution of gentian violet. 



The lowest filament is as yet sporeless ; some of its component segments are but half 

 the length of others, and separated by a narrower interval, indicating that fission and 

 interstitial elongation are proceeding in the filament. 



In the others, various stages of spore-formation are shown. The spores appear at 

 first as minute unstained points at or near the centre of the segments, the capsule of 

 tha spores preventing the penetration of the dye. When fully grown, the spores dis- 

 tend the wall or "sheath" of the bacillary cell, from which they are subsequently set 

 free, as shown at the highest part of the figure. In many of the sporulating segments 

 there is a minute second point, probably an abortive second spore, since the fully 

 developed spore in in most instances not strictly central, but nearer one end of the 

 spore-bearing segment, and this whether the latter presents a second point or not. 



The staining or the spores is best carried out by means of hot carbol fuchsin, and 

 the use of acid and methylene blue as described under the Tubercle bacillus. 



FIG. 2. ANTHBAX BACILLUS. 



Broth culture of 24 hours' growth, incubated at 87 C. Carbol fuchsin, washed in 

 acidulated water. 



Very few spores were found in the preparations, and none are present in the 

 filaments figured. The spore formation takes place only under the free access of gaseous 

 or atmospheric oxygen. The filaments adhere in tresses or strands. 



Active subdivision is taking place in the segments, the ends of which remain straight 

 or squarely cut. 



