PLATE XXI. 



FIG. i. -BACILLUS OF GLANDERS (BACILLUS MALLEIJ. 



From the growing edge of a culture on glycerin-agar of three days' growth, incubated 

 at 37C. 



Carbol fuchsine, washed in diluted acetic acid. The culture was made from a potato growth 

 of characteristic honey-yellow colour, raised from a glandered horse, the potato culture being 

 the second remove only from the original. 



The bacillus (which is very difficult to stain with certainty in the tissues) may be stained 

 in cover-glass films with aqueous solution of gentian violet (ten minutes), followed by washing 

 in i in 10,000 caustic potash solution and afterwards tap water ; or, by means ot alcoholic 

 solution of gentian violet for one hour, rapidly washed in water. The action of Loffler's 

 blue, for cover-glass films is uncertain. 



Somewhat slender rods of varying length and thickness, straight or slightly curved, the 

 shortest appearing as ovals, the longer as unsegmented filaments which are commonly less 

 deeply stained ; some of the filaments present were longer than those figured. Here and 

 there end-to-end pairs are met with. In some of the rods one or more minute, sharply- 

 defined, circular vacuoles are present. A few of the bacilli present a inoniliforin outline, but 

 none any distinct segmentation of protoplasm or " beading," as they may in the tissues. 



The organisms tend to cohere in clusters, some of the smaller of which are selected in the 

 illustration. 



FIG. c.-TUBERCLE BACILLUS. 



Pure culture on Luffler's blood-serum, incubated at 37 C., carried on from a growth on 

 glycerin-agar which was raised from the lymphatic gland of a guinea pig, inoculated from a 

 case of tubercular pleurisy in the human subject. 



The growth on the serum progressed slowly and took the form of a thin, white film in 

 which were sparsely scattered, thicker, more opaque areas. 



Stained with ca.'bol fuchsine warmed on the cover-glass, and treated with 25 per cent, 

 sulphuric acid in a watch-glass. If used in the cold, the dye should be allowed fifteen 

 minutes. 



The culture consists of straight and slightly-curved rods with rounded ends, and of varying 

 length, the shortest hardly more than oval. The bacilli tend to occur in small parallel 

 groups arranged in irregular lines, or set at .various angles to one another in away suggestive 

 of Chinese characters. They exhibit none of the segmentation, or " beading," so commonly 

 presented in phthisical sputum (see Plate XXX in the previous volume). 



Branching Forms. On the right are shown, from the same culture, two examples of the 

 filamentous forms, which recall the branched mycelium of the hyphal-fungi or moulds. 



The colouration of the bacilli (stained by Gram's method) is almost limited to the minute 

 spherical granules lying within the bacillary cell._ _ 



Branching forms are at times met with in phthisical sputum. 



