290 ON THE GERM THEORY OF PUTREFACTION 
as the bacteria of the urine, as is illustrated by the plants 6 and c, and here and 
there such buds were seen floating free in pairs such as /, but no bacteric move- 
ment was to be seen. This puzzled me at the time; but I afterwards found 
that it was no reason for surprise, and I shall hereafter have occasion to mention 
cases of bacteria of ordinary form and active movement in urine, assuming 
a motionless character and at the same time a very different appearance in 
other media. 
Although the proof already afforded of the identity of the Torula Ovalis 
with the filamentous fungus may appear sufficiently ample, yet, as the point is 
of extreme interest, I have been well pleased to obtain further confirmation of the 
fact while preparing this communication for the press. On the 9th of November, 
1873, I once more removed the test-tube containing the Pasteur’s solution from 
its cotton packing to see what change it might have undergone. I found about 
half of the original volume of the liquid still remaining unevaporated. It was 
still transparent, but it was now of a pale brownish-yellow colour, and the sedi- 
ment had a similar tint. A delicate incrustation existed on the interior of the 
glass, but did not reach up to the level of the liquid, and the gelatinous lumps 
had disappeared from the dried part above. Raising the test-tube cover with 
careful antiseptic precautions, I removed a few drops, taking up at the same 
time a little of the crust, which I detached from the side with the ‘ heated’ 
pipette ; and, after inoculating a glass of Pasteur’s solution with about half 
a minim, I proceeded to investigate the remainder. Under the microscope the 
solid constituent proved to be composed in the main of granular masses, looking 
like confused aggregations of the organism in an effete and degenerate state ; 
but projecting from the edges of these masses were plants and corpuscles, which, 
from their translucent and fresh appearance, made me hope that they were 
alive. The filaments closely resembled those seen in this glass a year and 
a quarter before, except that they were invariably very short, and the corpuscles, 
while sometimes in groups more or less resembling the original torula, were 
often of a more elongated form and strongly nucleated. During the first five 
days after the inoculation there was no distinct appearance to the naked eye 
of any growth taking place in the new glass of Pasteur’s solution. At the end 
of that time, however, thinking that a speck of delicate scum, which existed 
from the first, appeared slightly increased, I examined a portion microscopically, 
and found it to consist entirely of cells which appeared of new formation, some 
of them presenting transitional forms between the elongated bodies common 
in the test-tube and the constituents of the oval torula. The growth after- 
wards continued, both as a very delicate scum, and as a fine white deposit ; 
but its rate was extremely slow, and the product for the most part on a much 
