~ 
AND OTHER FERMENTATIVE CHANGES 201 
smaller scale than the original torula, and more resembling the elements found 
in the test-tube. 
Very different was the behaviour of the organism in unaltered urine. Two 
days after the inoculation of the Pasteur’s solution, I introduced half a minim 
of the liquid from the test-tube into a ‘ heated’ and covered glass, containing 
unboiled urine from a flask which had been charged on the first of March, but, 
though it had furnished the material for many successive experiments, retained 
its original characters unimpaired.’ For two days there was no appearance of 
growth ; but on the third day a small patch of scum, which had been the im- 
mediate result of the inoculation, was considerably increased in size, and had 
acquired a much coarser character, and several small detached specks of similar 
aspect were floating on the surface. The side of the glass was also sprinkled 
with minute particles like grains of white sand, often disposed in vertical streaks, 
while other similar granules were deposited at the bottom, the liquid retaining 
its briliant clearness. In short, the naked-eye appearances were an almost 
exact reproduction of those which resulted from the introduction of the rain 
drops into the original urine nearly two years before, and on applying the 
microscope to a portion of the scum taken up with ‘ heated’ pipette, I was 
delighted to find 1t composed exclusively of the Torula Ovalis in all its original 
beauty, the constituents cells pullulating freely, as shown at 7, Plate VII, which 
represents, for convenience of sketching, a small specimen of the groups, which 
were commonly much larger, like those of the yeast plant when in full activity. 
In some fields the cells were peculiarly large, as at m, and here and there, as at 
Zand n, a cell was somewhat longer than usual, just as occurs in Torula Cere- 
vistae ; but there was no appearance of filamentous growth. It was a torula 
pure and unmixed ; yet its identity with the Torula Ovalis and its distinction 
from the yeast plant were declared not only by the form and aspect of the cells, 
but still more by the fact that, just like the original specimen, it grew freely 
in non-saccharine urine, in which Torula Cerevisiae develops only with extreme 
difficulty. uy 
The organism, having thus, after many months of slow growth in the fila- 
mentous form in the altered Pasteur’s solution, recovered its purely toruloid 
and luxuriant habit in the medium in which it presented those characters at the 
outset, retained them when transferred to uncontaminated Pasteur’s solution. 
For having, with the touch of a ‘heated’ pipette, introduced a speck of the 
rapidly growing scum from the urine into a second glass of Pasteur’s solution, 
which had been charged along with the former six days before, but had hitherto 
’ The method by which this flask was prepared, and the mode of decanting into the experimental 
glasses, will be described in a later part of this paper. 
U2 
