294 ON THE GERM THEORY OF PUTREFACTION 
elapsed. I then found the sour liquid blacker than ever, and further reduced 
by evaporation, the only other change visible to the naked eye being that the 
same low white mould had grown again in small amount upon the side of the 
glass. Finding that it still retained the same characters under the microscope, 
I hoped that by transferring it to a saccharine solution I might get it to repro- 
duce the Torula Cerevisiae, just as I had got back the Torula Ovalis by placing 
its filamentous form in fresh urine. Accordingly, having taken up a portion of 
the mould with a ‘ heated’ knife, I introduced a morsel of it into a ‘ heated’ 
and covered glass containing freshly prepared Pasteur’s solution, and placing 
the remainder in a drop of water between plates of glass, made a further examina- 
tion with the microscope. a, in Plate VIII represents a fructifying filament, 
the segments of which are some of them in the form of a moniliform chain of 
spores, while others present a transverse line indicating tomiparous division 
into gemmae, and one has given off a conidial bud, the last being an appearance 
comparatively rarely seen in this fungus when first removed from the wine- 
glass. But on examining again, after fifteen hours, the same specimen, which 
had been kept in a moist atmosphere to prevent evaporation, I found free spores 
in considerable numbers about the filament previously sketched, and the fila- 
ment itself was studded with numerous fresh conidial buds, as shown in outline 
at a,, the one previously present having dropped off. The great rapidity with 
which this conidial budding took place under the influence of the water is further 
indicated by the sketch at a, taken only two hours later, where all the buds 
present at the former examination are seen to have either grown larger or to 
have dropped off, while several fresh ones have made their appearance. 
This abundant formation of conidia in the new medium increased my hopes 
that I should get back the Torula Cerevisiae in a saccharine fluid. This hope, 
however, was doomed to disappointment. So far from the organism exhibiting 
in the glass of Pasteur’s solution a toruloid development, it assumed there the 
opposite condition of a filamentous growth, in which any appearance of conidial 
formation was a rare occurrence. 0, c, and d in Plate VIII represent sprouting 
conidia, ¢ a very young plant, and / the extremity of a filament. The entire 
distinction of this fungus from the yeast plant was further shown physiologically 
by the fact that it grew extremely slowly in the saccharine liquid, and failed 
to cause any evolution of gas in it, though kept under observation more than 
two months. I was thus led to conclude that this oidium had been merely an 
accidental concomitant of the yeast plant, having sprung, perhaps, from one 
of the adventitious filamentous plants noticed during the first few days in the 
glass, and having survived the chemical changes in the fermenting liquid under 
which the yeast plant itself had succumbed. 
