296 ON THE GERM THEORY OF PUTREFACTION 
disposed in pairs, as shown at e, Plate IX, or of plants of a most imperfect 
description, consisting of cells of a similar character to the free ones, or slightly 
more elongated, connected end to end, and often producing conidial buds, as in 
the specimen figured at d in the same plate. 
On the following day the difference between the two glasses was still more 
marked. The filamentous plants in the Pasteur’s solution had considerably 
increased, but those in the urine had almost all fallen to the bottom, their places 
being taken by abundant specks and streaks of granular aspect, and even the 
few plants that still remained adhering had lost their purely filamentous char- 
acter and had become granular. There were also little patches of scum upon 
the urine, whereas the surface of the Pasteur’s solution presented only some 
floating filamentous plants. I removed a portion of the scum with ‘heated’ 
pipette, and submitted it to the microscope, and found it to consist exclusively 
of free oval cells, like those seen in the granular specks the day before, as shown 
in outline at f. In the course of the next twenty-four hours all appearance of 
filamentous growth disappeared from the urine; but while the liquid, which 
was now for the first time observed to have a slightly offensive smell, had become 
unsuited for that mode of development of the organism, it had stimulated the 
corpuscular form in a most remarkable manner, the scum having increased 
with amazing rapidity. Thus, between 8 p.m. on the 24th and 5.30 a.m. on the 
25th, it grew from a loose patch, about half an inch in diameter, to a dense film 
that covered almost the entire surface of the liquid in the urine-glass, and eight 
hours later, the cell growth had been so great that the scum had become pushed 
up upon the glass to about a quarter of an inch above the level of the liquid, 
while the urine was rendered cloudy by the subsidence of detached cells. In 
the course of the afternoon the liquid had become turbid throughout, and the air 
in the glass shade was still more decidedly offensive ; yet, under the microscope, 
the only organism discoverable was that represented by the pairs of cells before 
described, so that we have here another clear example of fermentative change 
of putrefactive character induced in urine by other agency than bacteria. 
Samples of the cells are given in g, Plate IX, where they are seen to resemble 
those of d and e in having vacuoles, but no nuclei, merely, in some cases, incon- 
spicuous granules. In g is also given in outline a portion of the scum, showing 
how densely packed the constituent cells were, corresponding with the remark- 
able naked-eye appearance, which was that of a dense white layer, like a film 
of paraffin. 
On the same day (August 25), I introduced a small portion of this scum 
into a second glass of urine, prepared along with the former one fifteen days 
previously, but as yet retaining its brilliant clearness, and in other respects also 
