AND OTHER FERMENTATIVE CHANGES 201 
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introduction. At 9.50 p.m. the nuclei were found more conspicuous and 
altered in position, but there was as yet no change of form in the cells. Early 
next morning I found that the cells generally were sprouting ; but it happened 
that those which I had drawn had shifted their position slightly, so that I could 
not distinguish them in their now altered shape from others in their vicinity, 
but I selected two groups for further observation, represented at 6, and c,, 
sketched at 1.30 and 1.35 a.m. respectively. It will be observed that, while 
this early stage of germination has changed them from the spherical to an oval 
form, they still retain their nucleated character. Five hours later, growth had 
advanced in both groups so as to give the appearance represented at b, and c,, 
In both groups the nuclei have almost disappeared, while the sprouts have 
much increased ; and in c,, while the highest of the three cells has produced 
a short filament, the lowest has formed two oval vacuoled cells, and the 
other, after growing an oval cell, has gone on to the development of a short 
filament. 
After four more hours had passed, I was rejoiced to find the experiment 
crowned with complete success. The longer sprouts of c, had become extended 
to threads of considerable length, as represented in c,; while the progeny of the 
other original cell was in the form of pairs of oval vacuoled bodies destitute of 
nuclei, exactly resembling the constituents of the first scum, or of the granular 
deposits which accompanied the woolly tufts on the first urine-glass. And just 
as in that glass, at an early period, some plants exhibited the filamentous 
others the corpusclar form of growth, so was it with the offspring of the three 
spherical cells whose development we have followed. 
Such was the effect of uncontaminated urine upon this organism. After- 
wards, however, as the liquid gradually became vitiated under its fermenting 
influence, the filamentous form of growth which first appeared began to give 
place again to the corpuscular, a change which the ‘ glass garden’ afforded oppor- 
tunity of watching with perfect precision. c, shows the lower of the two fila- 
ments of c, at 5.50 p.m. on the same day, represented on a smaller scale. It will 
be observed, that, while the filament has increased considerably in length, it 
exhibits a tendency to break up into segments, and here and there along its course 
it has produced oval corpuscles. And a further progress of the same altera- 
tion of habit is exhibited in c,, where the same filament is again sketched on the 
same scale after the lapse of ten hours more, viz. at 3.50 a.m. on the 13th of Sep- 
tember. The filament has only increased very slightly in length, but the terminal 
portion has broken up into segments, and assumed a zigzag form in consequence, 
while a multitude of corpuscles have been produced in the course of the filament, 
partly by budding of the segments of the thread, and partly by the pullulation 
