AND OTHER FERMENTATIVE CHANGES 305 
that power in fresh urine, implying that this secretion, when in a perfectly un- 
altered condition, is a still more favourable medium for the organism, permitting 
a degree of recovery which was impossible in Pasteur’s fluid. 
The last fact which I have to mention regarding this plant, is its behaviour 
in an albuminous liquid. This medium, which also proved valuable in experi- 
ments to be described in a later part of this paper, was prepared on the same 
principle as the unboiled urine, by taking the material uncontaminated from its 
natural receptacle, by aid of antiseptic measures. An egg, known to have been 
laid within the last twenty-four hours, was steeped for a while? in a solution of 
carbolic acid in twenty parts of water, to destroy any organisms adhering to 
the shell, and was then broken in a fine spray of carbolic-acid solution of the 
same strength, and about an ounce of the white of the egg was introduced into 
a flask containing ten ounces of water, which had been boiled and allowed to 
cool, the air which entered during cooling having been filtered of dust by a mass 
of cotton-wool tied tightly over the mouth of the vessel before boiling. The 
flask was agitated occasionally during the next twenty-four hours, to promote 
diffusion of the albumen in the water, after which the liquid was passed through 
a boiled filter placed in a ‘heated’ funnel, protected with a ‘heated’ glass 
cover, under a large glass shade.? It was thus cleared of the shreddy residue 
of the white of egg, and also of the opaque floccules resulting from the action of 
the carbolic-acid spray upon the albumen, and was obtained of crystal clearness in 
the ‘heated’ flask into which it was received, and in which it was kept pro- 
tected from dust by a ‘ heated’ glass cap and a glass shade. <A ‘ heated’ wine- 
glass, provided with cover and glass shade as usual, being charged with some 
of this liquid, I inoculated it with a little of the toruloid scum from the second 
wine-glass on the 3rd of September. The result was a corpuscular development 
of a delicate inconspicuous character, the growth proceeding so slowly that the 
little patch of scum, in which alone any increase was observed, had not 
doubled its diameter in ten days. I now introduced with a ‘heated’ needle 
a little piece of the fungus, in the filamentous form, from the first glass 
of Pasteur’s solution. This retained the filamentous mode of growth in the 
* The actual time was much longer than I had intended, viz. two days. A subsequent experi- 
ment, in which one hour and twenty minutes was the period of immersion, was equally successful. Even 
after the two days of the present occasion, the carbolic acid did not seem to have affected the albumen, 
which was free from coagulation to the surface. 
* This was a most troublesome procedure to carry out. I afterwards simplified the process very 
much, so as to dispense with both the spray and the filter, extracting the albumen with ‘ heated ’ pipette 
passed into a hole made in the carbolized shell with ‘ heated’ forceps, a piece’ of carbolized cotton- 
wool being wrapped round the pipette and egg to prevent entrance of dust, filtration of the mixture of 
albumen and water being effected by decanting through a boiled syphon, which had a piece of sponge 
tied over the end in the flask. 
LISTER I D4 
