320 A FURTHER CONTRIBUTION TO THE 
The artificial milk and Pasteur’s solution were turbid the day after inocu- 
lation: and in the former, which I examined microscopically, were seen active 
bacteria of extreme minuteness, looking like mere pairs of granules, which on 
the following day had given place to others of larger size and of the same sort 
of characters as those of the milk, as shown at m, Plate XI. Similar bacteria 
were also seen at this time in the Pasteur’s solution. But neither then nor at 
any subsequent period was there any viscidity of the general liquid in either 
of these glasses, implying that the viscid substance was no essential appen- 
dage of the organism, but the result of its fermentative action upon particular 
materials. 
It is, however, to be added that in the course of the next month a deposit 
occurred upon the sides of both these glasses such as I never saw under any 
other circumstances, constituting a film which, in the artificial milk, resembled 
coagulated fibrine in its toughness, and in the Pasteur’s solution was tenacious 
though not viscid, as if the motionless bacteria which constituted the deposit 
in each case had been glued together by a minute quantity of some intervening 
substance. 
The next observation which I have to record has reference to the origin 
of bacteria. It will be remembered that a filamentous fungus made its appear- 
ance on the interior of the first milk-glass six days after its exposure. The 
growth continued to spread, and by the tenth day, as it had a bloom indicating 
probable fructification, I scraped off a small portion from the glass by means of a 
tenotomy knife washed with strong carbolic solution and dried in the flame, 
and examined the specimen in a drop of water with the microscope. It proved 
to be a fungus of great beauty composed of very delicate branching filaments 
(a, Plate XI), bearing spores (conidia) often septate, characterized by a raw 
sienna tint (c, Plate XI) which was often distinctly seen to be confined to an 
external envelope, affording, what is unusual with fungi of such minuteness, the 
means of definite recognition, and of ascertaining with precision the three modes. 
of germination above alluded to (see pp. 302, 309). Many of the spores were 
seen to have produced thick sprouts to form young plants. Of these d has been 
sketched because it happened that, while part of the brown envelope had been 
consumed in the process of germination, a portion still remained for identifica- 
tion. Other spores were observed in toruloid pullulation, as is seen at e in 
a mass still connected with the parent filament, and at g in a free and septate 
spore, while 7 was either a spore multiplying by pullulation, or a young plant 
of a brown colour. For here and there young plants were seen like 0 retaining 
the brown investment of the spores; and hence, as a dark-coloured coat of 
threads and spores is the special character of the order Dematiei among hypho- 
