326 A FURTHER CONTRIBUTION TO THE 
one does, an elongated state of some of the segments of the chain, thus forming 
connecting links between the leptothrix and the widely different spirillum-like 
specimens such as e. Next day the same sort of appearances were again seen, 
and an observation made on the previous day was confirmed, viz. that vacuoles 
were present in the thicker specimens. This is well shown in the sketches 
g and h, in all of which there is also a further deviation from the type which 
has been lately held to be invariable in the entire group of bacteric organisms, 
and from whence the name schizomycetous, as applicable to a totally distinct 
order of fungi, has been derived, that is to say these bacteria, instead of multi- 
plving by transverse fission, are plainly increasing by pullulation, that is to say, 
by shooting out buds after the fashion of the yeast plant ; and it will be observed 
that these sprouts are by no means always in a line with the long axis of the 
organism from which they spring. Yet that they really were the same bac- 
terium was evident, not merely from transitional forms, but from specimens 
such as f/, in which in one and the same chain we have the leptothrix character 
combined with the long and thick vacuoled and pullulating organism. Similar 
observations were made on the following day ; and now even the smallest and 
most bacteriform specimens sometimes exhibited a minute vacuole, as is shown 
at 7. These appearances did not startle me as much as they would have done 
had I not seen something almost exactly similar in an earlier part of the 
investigation, though in another species of bacterium under totally different 
circumstances. 
Thinking it worth while to try how this organism would behave if trans- 
ferred from the urine to Pasteur’s solution, I used for that purpose some of 
the old February stock, still perfectly bright, inoculating on the 18th. Next 
day the fluid was distinctly nebulous as examined before a candle, and under 
the microscope I found motionless bacteria, not numerous, but obviously of 
new formation from the delicacy of their aspect, represented at k, in Plate XII, 
where they are seen to be of considerable thickness and length of the segments, 
which present a curious alternation of lightness and darkness in their substance. 
Though a pair and three are given in the sketch as well as a single one, solitary 
individuals were much the most frequent. Such was the appearance twelve 
hours after inoculation, but when twelve more hours had expired a very great 
change had taken place. Not only were the bacteria much more numerous, 
but very much smaller ; and instead of being commonly single, were invariably 
double, having in fact the ordinary appearance of minute bacteria (see Plate XII, 
/), and to complete the metamorphosis some of these bacteria were seen swimming 
actively in ordinary bacteric fashion. Two days later the liquid was consider- 
ably increased in opacity and I was struck with what I had never seen before 
