GERM THEORY OF FERMENTATIVE CHANGES Bei 
in Pasteur’s solution, a sort of dirty or dingy appearance, as if a very small 
quantity of ink had been mingled with the liquid, and the deposit at the bottom 
of the glass, which was white on the previous day, had now the same dingy 
cast. Under the microscope the bacteria appeared much as on the last occasion, 
except that some were even more minute than any then were, so that it was 
impossible to say, except by their movements, that they were anything more 
than mere granules (see m, in Plate XII). At the same time active movement 
was more frequent than before. 
I now thought it well to ascertain whether these minute and active bacteria 
would reproduce in urine the same sort of organism as that which we could 
not but believe to have been their parents in that fluid. On this occasion, 
having no more of the fresh urine, I adapted a syphon to a flask which was 
prepared on the 1st of March, and had furnished the material for numerous ex- 
periments, yet retained its original brilliancy as well as odour unaltered, was 
distinctly acid to litmus, and displayed no organisms under the microscope. 
Twelve hours after the inoculation on the 21st the liquid was already manifestly 
nebulous, and on examination with the microscope bacteria were found, four or 
five in every field, differing from those that had been introduced in being very 
rarely double but long and large and often curved (vide Plate XIII, a), having thus 
returned to a considerable extent to the condition before seen in urine, but now 
differing from their former state in that fluid in frequently exhibiting character- 
istic though languid movements. After twelve hours more the previous con- 
dition in urine was still more closely approximated by greater length in the 
segments, as illustrated by 5, sketched because it happened to be at rest, though 
by no means having the longest unbroken segments that were observed. I now 
inoculated from this glass of urine another (Urine III) that had been decanted 
on the same day and had remained till then unchanged ; and twelve hours 
afterwards I sketched from this second glass the magnificent example of un- 
jointed spirilliform organism represented at #. At the same time languid move- 
ment was seen in many specimens. 
To complete the history of the behaviour of this organism in urine it may 
be added that, after the lapse of another fortnight, the bacteria in this glass 
were found again motionless and comparatively small, scarcely differing in 
appearance from those originally seen in the sour milk (vide foot of Plate XIII). 
With the view of determining precisely the identity of the minute organism 
in the Pasteur’s solution with the large one in urine, I stocked as follows, on 
the 21st of August, a ‘ glass garden’ consisting of a massive piece of plate-glass 
excavated by the lapidary into a broad and deep ditch around a central island, 
the ditch to serve asa reservoir of air. This glass, together with a thin covering 
