PEACTICAL PHYSIOLOGY. 



[VI. 



(a.) Dilute the solution in a test-tube and observe its spectrum, 

 noting that a stronger solution is required than with Hb0 2 , to show 



the absorption-bands. Two absorp- 

 tion-bands nearly in the same posi- 

 tion as those of Hb0 2 , but very 

 slightly nearer the violet end (fig. 24, 

 3). Make a map of the spectrum 

 and bands. 



(&.) The bands are not affected by 

 the addition of a reducing agent, 

 e.g., ammonium sulphide or Stokes's 

 fluid. Add these fluids to two 

 separate test-tubes of the solution 

 of COHb, and observe that the two 

 absorption bands are not affected 

 thereby. There is no difference on 

 |C shaking the solution with air, as the 

 compound is so very stable. 



(c.) To a fresh portion of the solution of 

 carbonic oxide haemoglobin add a 10 per 

 cent, solution of caustic soda and boil = 

 cinnabar-red colour. Compare this with 

 a solution of oxy-haemoglobin similarly 

 treated. The latter gives a brownish-red 

 mass. 



(d.) Dilute i cc. of blood with 20 cc. of 

 r. i. 34). 

 " first 

 presently 



A. Slip- red. When allowed to stand, flakes form 

 and settle on the surface. Normal blood 

 gives a dirty brown colouration. 



(e.) Non-Eeduction of HbCO. Repeat the above experiment (VI. 8) with 

 carbonic oxide haemoglobin, and note that this body is not reduced by putre- 

 faction. Or seal up the blood in a tube. 



12. IV. Acid-Haematin. 



(a.) To diluted defibrinated blood add a few drops of glacial acetic 

 acid, and warm gently, when the mixture becomes brownish owing 

 to the formation of acid liematin. 



(b.) The spectrum shows one absorption-band to the red side of 

 I) near C (fig. 28, 5), and there is considerable absorption of the 

 blue end of the spectrum. 



The single band is not affected by ammonium sulphide or 

 that sulphur is precipitated if Arn^S is used, 

 e Tiu TO made alkaline hsemochromogen is formed. 

 NJl.-^-\i. acetic acid alone be used to effect the change, observe 

 that-only one absorption-band is seen. 



FIG. 27. Hsematoscope of Hermann. F. 

 Glass plate; C. Piston-like tube, 



for holding surplus fluid. 

 p*ort. 





