VI.] 



THE COLOURED BLOOD CORPUSCLES 



13. Acid-Hsematin in Ethereal Solution. 



(a.) To undiluted defibrinated blood add glacial acetic acid, 

 which makes the mixture brown. Extract with ether, shake 

 vigorously, and a dark-brown ethereal solution of hsematin is 

 obtained. Pour it off and 



(/>.) Observe the spectrum of this solution four absorption 

 bands are obtained. The one in the red between C and D, corre- 

 sponding to the watery acid-hsematin solution ; and on diluting 

 further with ether a narrow faint one near D, one between D and 

 K, and a fourth between b and F (fig. 28, 5). The last three 

 bands are seen only in ethereal solutions, and require to be looked 

 for with care. 



14. V. Alkali-Hsematin. 



(a.) To diluted blood add a drop or two of solution of caustic 

 potash, and warm gently. The colour changes to a brownish-green, 

 and the solution is dichroic. Or use a solution of acid-hsematin ; 

 neutralise it with caustic soda until there is a precipitate of 

 hsematin ; on adding more soda and heating gently, the precipitate 

 is re-dissolved, and alkali-ha' matin is formed. 



(b.) Shake (a.) with air to obtain oxy-alkali-haematin. Observe 

 its spectrum, one absorption-band just to the red side of the 1) 

 line. It is much nearer D than that of acid hsematin (fig. 28). 

 Much of the blue end of the spectrum is cut off. 



Red. Orange. 



Yell 



Green. 



Blue. 



A a 



90 100 uo 

 E F 



5. Hsematin in ether with sulphuric 





FiG. 28. Spectra of Derivatives of Haemoglobin. 



acid ; 6. Hamiatin in an alkaline solution ; 7. Reduced hscniatin. 



15. Reduced Alkali-Hsematin or Hgemochromogen. 



(<i.) Add to a solution of alkali-hsematin a few drops of ammonium 

 sulphide and warm gently. Note the change of colour = reduced 



