Staining Bacteria 



175 



'have been abandoned. The affinity between the bacteria 

 and the anilin dyes is peculiar, and in certain cases can be 

 used for the differentiation of species. 



The best anilin dyes made at the present time, and those 

 which have become 

 the standard for all 

 bacteriologic work, 

 are made in Ger- 

 many by Dr. Grii- 

 bler, and in or- 

 dering stains the 

 name of this man- 

 ufacturer should be 

 specified. 



Readers interest- 

 ed in the biochemis- 

 try of the subject 

 will do well to refer 

 to the excellent 

 papers by Arnold 

 Grimme,* upon 

 "The Important 

 Methods of Stain- 

 ing Bacteria, etc.," 

 and Marx,f upon 

 " The Metachro- 

 matic and Babes- 

 Ernst Granules." 



In this work 

 special methods for 

 staining such bac- 

 teria as have pecu- 

 liar reactions will 

 be given together 

 with the descrip- 

 tion of the particular organisms, general methods 

 being discussed in this chapter. 



Preparations for General Examination. For bacterio- 

 logic purposes thin covers (No. i) are required, because 

 thicker glasses may interfere with the focussing of the oil- 

 immersion lenses. The cover-glasses must be perfectly 



* "Centralbl. f. Bakt.," etc., Bd. xxxn, Nos. 2, 3, 4, and 5, 1902. 



f Ibid., xxxii, Nos. 10 and u, p. 108, 1902. 



Fig. 33. Apparatus for keeping objects 

 under microscopic examination at constant 

 temperatures (Nuttall). 



only 



