The Hemolytic Amboceptor 



333 



The tubes are again stood in the thermostat, where they 

 are permitted to remain for an hour, when the readings are 

 taken and carefully noted. After this the rack and all the 

 tubes are placed in the ice-box until twenty-four hours' old, 

 when the final readings are taken and the conclusions are 

 reached. 



As a rule, the readings taken after the second hour of in- 

 cubation and those taken after twenty-four hours correspond. 



A valid test should show the following: 



Test Controls. 



Tubes. 

 I. 



2. 



3- 

 4- 



6. 



8. 

 9- 



No hemolysis in syphilis. 

 Complete hemolysis. 



Hemolysis in health. 



No hemolysis (this is the standard of comparison). 

 Complete hemolysis. 



No hemolysis, as a rule. 



Fig. 101. A typical positive Wassermann reaction with the recom- 

 mended controls as it appears after standing twelve hours. Corpus- 

 cular sedimentation without hemolysis is seen in tubes i, 3, and 9; 

 complete hemolysis in the others. 



In the tubes in which hemolysis takes place the change is 

 very marked. The hemoglobin dissolves out of the corpus- 



