422 Hydrophobia, Lyssa, or Rabies 



Sat. ale. sol. methylene violet 10 c.c. 



Sat. ale. sol. fuchsin 7 drops 



Sterile water 40 c.c. 



The smears of cerebellum or hippocampus are fixed with 

 absolute alcohol and ether and the stain poured on, heated, 

 poured back into the bottle, again poured on, heated and 

 poured back into the bottle, this being done three times, each 

 time for about half a minute. Then wash in water, blot, and 

 examine. To examine, a nerve-cell is found with the low 

 power and then examined with the high power. The Negri 

 bodies are brick red. The stain soon fades. Smears kept 

 for any length of time lose the staining reaction. 



Antirabic Serum. Babes and Lepp* thought that the 

 serum of animals that have received repeated injections of 

 the crushed nervous tissue of rabid animals was neutralizing 

 or destructive to the rabies virus in vitro, called it "anti- 

 rabic serum," and believed that it conferred a defensive 

 power upon other animals. Marie t found it to be a simple 

 neurotoxic serum and inert in its. action upon the virus. 



* "Ann. de 1'Inst. Pasteur," 1889, m. 



t " Compt.-rendu Soc. Biol.," t. i,vi, June 18, 1904, p. 1030. 



