444 Chancroid 



and mixed with fresh rabbits' blood drawn under aseptic 

 precautions, in the proportion of two-thirds agar to one- 

 third blood, and slanted while in a fluid state. At a later 

 period tubes of rabbits' blood-serum uncoagulated, also 

 rabbits' blood bouillon, one-third blood to two-thirds bouil- 

 lon, were used, and gave equally satisfactory results. By 

 employing small tubes of freshly drawn human blood pure 

 cultures were obtained in several instances from genital 

 lesions direct without any special cleansing of the ulcerated 

 surface. This, I believe, is the best medium for obtaining 

 cultures from a source open to contamination, the fresh 



Fig. 139. Culture from ulceration on monkey resulting from inocu- 

 lation of culture from a case of chancroid of finger, first generation. 

 Stained with carbol-fuchsin and briefly decolorized by alcohol. Cul- 

 ture of twenty-four-hours' growth in rabbit's bouillon. X 1500 (Davis). 

 (Photomicrograph by Mr. L. S. Brown.) 



blood apparently inhibiting to a certain extent the growth 

 of extraneous organisms." 



No growth takes place upon ordinary culture-media under 

 either aerobic or anaerobic conditions. 



Cultures are best obtained by puncturing an unopened 

 bubo with a sterile needle and planting the pus directly and 

 immediately upon the special medium which should have 

 been warmed in the incubator so that the pus is not chilled. 

 In this way pure cultures may be secured, which are difficult 

 to get from the soft sore itself. 



Colonies. The colonies appear upon the appropriate 

 media in about twenty-four hours, and attain their complete 



