Mosquitoes and Malarial Fever 545 



after staining and washing, and mounted in Canada balsam 

 under a cover-glass. 



A more certain and more elegant manner of showing the 

 parasites in infected mosquitoes is by pulling off the legs and 

 wings and then embedding the insect in paraffin and cutting 

 serial longitudinal vertical sections. 



To infect mosquitoes and study the development of the 

 malarial parasites in their bodies, the insects should be 

 bred from the aquatic larva in the laboratory, to make sure 

 that they do not already harbor parasites. The mosquitoes 

 are allowed to enter a small cage made with mosquito netting, 

 and are taken to the bedside of the malarial patient, against 

 whose skin the cage is placed until the insects have bitten 

 and distended themselves with blood, when they are taken 

 back to the laboratory and kept as many days as may be 

 desired, then killed and sectioned. In this way, remember- 

 ing that the entire mosquito cycle of development takes 

 about a fortnight, any stage of the cycle may be observed. 



35 



