Cultivation 779 



cavity; also by Levy and Steinmetz,* who found that 

 Staphylococcus pyogenes aureus was also capable of provok- 

 ing suppurative orchitis. However, the diagnosis is certain 

 if a culture of the glanders bacillus be secured from the 

 pus in the scrotum. 



As the purulent discharges from the noses of horses and 

 other large animals commonly contain very few bacilli, 

 their detection by the use of the guinea-pig inoculation is 

 much simplified. 



For the diagnosis of the disease in living animals, sub- 

 cutaneous injections of mallein (q. v.) are also employed. 



McFadyenj was the first to recommend agglutination of 

 the glanders bacillus by the serum of supposedly infected 

 animals as a test of the existence of glanders. The subject 

 has been somewhat extensively tried and officially adopted 

 by the Prussian government. Moore and Taylor, J in a recent 

 review and examination of the test, conclude that it is easier 

 and quite as accurate as the mallein method and is applicable 

 in cases where fever exists. The maximum dilution of 

 normal horse-serum that will macroscopically agglutinate 

 glanders bacilli is i : 500, but occurs in very few cases. The 

 maximum agglutinative power of the serum of diseased horses 

 not suffering from glanders is not higher than that of normal 

 serum. The diagnosis is usually not difficult to make, but 

 requires much care. Cultures of the glanders bacillus some- 

 times unexpectedly lose their ability to agglutinate. 



The diagnosis of glanders by means of the complement- 

 fixation method has been tried with glittering results by 

 Mohler and Eichhorn. 



Cultivation. The bacillus is an aerobic and optionally 

 anaerobic organism, and can be grown in bouillon, upon 

 agar-agar, better upon glycerin agar-agar, very well upon 

 blood-serum, and quite characteristically upon potato. The 

 optimum temperature is 37.5 C. 



Colonies. Upon 4 per cent, glycerin agar-agar plates 

 the colonies appear upon the second day as whitish or pale 

 yellow, shining, round dots. Under the microscope they 

 are brownish yellow, thick and granular, with sharp borders. 



Bouillon. In broth cultures the glanders bacillus causes 



* "Berliner klin. Wochenschrift," March 18, 1895, No. n. 

 t "Jour. Comp. Path, and Therap.," 1896, p. 322. 

 J "Jour. Infectious Diseases," iv, 1907, p. 85, supplement. 

 "Report of the Bureau of Animal Industry," 1910. 



