780 



Glanders 



turbidity, the surface of the culture being covered by a 

 slimy scum. The medium becomes brown in color. 



Gelatin is not liquefied. The growth upon the surface is 

 grayish white and slimy, never abundant. 



Agar-agar. Upon agar-agar and glycerin agar-agar the 

 growth occurs as a moist shining layer. 



Blood-serum. Upon blood-serum the growth is rather 

 characteristic, the colonies along the line of inoculation 

 appearing as circumscribed, clear, transparent drops, which 

 later become confluent and form a transparent layer un- 

 accompanied by liquefaction. 



Fig. 254. Culture of glanders bacillus upon cooked potato (Loffler). 



Potato. The most characteristic growth is upon potato. 

 It first appears in about forty-eight hours as a transparent, 

 honey-like, yellowish layer, developing only at incubation 

 temperatures, and soon becoming reddish-brown in color. 

 As this brown color of the colony develops, the potato for a 

 considerable distance around it becomes greenish brown. 

 Bacillus pyocyaneus sometimes produces somewhat the same 

 appearance. 



Milk. In litmus milk the glanders bacillus produces acid. 

 A firm coagulum forms and subsequently separates from 

 the clear reddish whey. 



Metabolic Products. The organism produces acids and 

 curdling ferments. It forms no indol, no liquefying or pro- 



